Isolation, partial purification, biochemical characterization and detergent compatibility of alkaline protease produced by Bacillus subtilis , Alcaligenes faecalis and Pseudomonas aeruginosa obtained from sea water samples
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منابع مشابه
Isolation, Purification and Characterization of a Thermophilic Alkaline Protease from Bacillus subtilis BP-36
The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...
متن کاملisolation, purification and characterization of a thermophilic alkaline protease from bacillus subtilis bp-36
the goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native iranian microorganisms. at the end of screening program, a bacillus subtilis bp-36 strain producing thermophilic alkaline protease was isolated from a hot spring in ardebil province. the target enzyme was purified using a one-step aqueous two-phase systems (atps) prot...
متن کاملPurification and Characterization of Alkaline Protease Produced by a Mutant Strain of Bacillus Subtilis
The present study describes the production, purification and characterization of alkaline protease from mutant strain of Bacillus subtilis EMS-8. The enzyme was purified using ammonium sulphate precipitation which gave 2.64 fold purification with 81.5% yield at 70% saturation. The molecular weight of the enzyme was determined using SDS-PAGE and it was found to be 25 KDa. The optimum pH of enzym...
متن کاملPurification and characterization of laundry detergent compatible alkaline protease from Bacillus cereus
An alkalophilic bacterium, Bacillus cereus produced an extracellular alkaline protease, which was found to be active at high temperature and pH range, suitable for commercial laundry detergents. B. cereus protease was partitioned in different aqueous two-phase systems such as PEG/dextran, PEG/potassium phosphate, PEG/sodium citrate, PEG/magnesium sulphate and PEG/sodium dihydrogen phosphate and...
متن کاملPurification and Kinetic Parameters Characterization of an Alkaline Protease Produced from Bacillus subtilis through Submerged Fermentation Technique
This paper reported the purification and kinetic parameters characterization of an alkaline protease produced from Bacillus subtilis through submerged fermentation process using rice husk as growth supporting substrate (by-product of rice industry) collected from Pearl Rice Mill Hafiz Abad, Faisalabad. Bacillus subtilis was cultured in fermentation medium under some pre-optimized growth conditi...
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ژورنال
عنوان ژورنال: Journal of Genetic Engineering and Biotechnology
سال: 2018
ISSN: 1687-157X
DOI: 10.1016/j.jgeb.2017.10.001